: Utilizing The computer software on your qPCR thermocycler, establish at which cycle the fluorescence intensity crosses a user‐specified threshold. Utilizing favourable and detrimental controls as benchmarks, go with a threshold that Obviously distinguishes amplification from non‐amplification. To keep up RNA integrity, cells and tissues are first lysed by incubation https://www.pinterest.com/pin/993958580252568563/
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