Multi-tag pyrosequencing has become a key method in the analysis of microbial community composition. However. it is well known that kinetic bias during the initial PCR amplification of such microbial communities can dramatically distort amplicon abundance prior to downstream emulsion PCR and pyrosequencing. Here we present a simple protocol combining length-heterogeneity PCR fingerpri... https://www.diegojavierfares.com/flash-choice-Little-Mermaid-Ariel-Girls-Purple-Costume-Tiara-amazing-save/
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