Abstract CRISPR-associated (Cas) transposases (CAST) are RNA-guided systems capable of programmable integration of large segments of DNA without creating double-strand breaks. Engineered Cascade CAST function in human cells but are challenging to deploy due to the complexity of the targeting components. Unlike Cascade. which require three Cas proteins. https://www.allfixelectricales.shop/product-category/fridge-ice-bucket-anti-twist-pin/